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To overcome the problems of free enzyme application as catalysts in chemical  reactions, i.e. high costs of isolation and purification processes, high  sensitivity to process conditions, insufficient enzyme stability etc., a  different immobilization techniques are to be used. Immobilization to/within  solid support improves enzyme stability decreasing its denaturation. This  paper deals with hydrogels of N-isopropylacrylamide and itaconic acid with  incorporated nonionic surfactants (Triton X-100, Brij 30 and Tween 80)  synthesized in distilled water at room temperature by free radical  polymerization. These hydrogels were used as supports for immobilization of  enzyme, lipase from Candida rugosa by post-entrapment method. The aim was to  investigate the effect of the nonionic surfactants on the lipase binding  capacity, as well as on its hydrolytic activity. In order to characterize the  obtained hydrogels FT-IR analysis has been performed. Further, the swelling  behaviour of these samples in buffer solution of pH 6.80 has been  investigated. The dynamic - mechanical properties of hydrogels and detailed  have been studied, too. The immobilized lipase showed somewhat reduced  hydrolytic activity, as compared to the activity of free lipase as well as in  comparison to the lipase immobilized to the reference sample (sample  synthesized under the same polymerization conditions, but in the absence of  surfactants). It was concluded that the addition of surfactants increased the  hydrogel mesh size. The surfactant addition did not affect the dynamic -  mechanical properties of the investigated hydrogels. The largest percentage  of specific activity and yield of activity were presented by the reference  sample, too. It is obvious that the absence of surfactants charged groups has  no influence on the lipase binding capacity, and the obtained activity yields  are to be expected

hemijska industrija

Immobilization of lipase from Candida rugosa into copolymer hydrogels of poly(N-isopropylacrylamide-co-itaconic acid) synthesized in the presence of surfactants