English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

The genuineness of a hybrid is one of the most important characteristics of good quality seed. In order to identify pure hybrid and pollen shedders/ offtypes an investigation was performed to identify an ideal SSR marker. 58 primer pairs were screened to identify the specific marker associated with each hybrid and parental lines. Hybrid KBSH-44 could be clearly identified by using ORS 309 and ORS 170, based on the banding pattern resolved on polyacrylamide gel (6%). The complementary banding pattern of both parents made a way to identify the hybrid. ORS 309 amplified allele size at 250 bp was specific to female parent (CMS-17A) and 230 bp was specific to male parent (RHA 95- C-1). These two bands of allele size 230 and 250 bp were found only in hybrid KBSH-44. Another SSR primer ORS 170 was able to distinguish the hybrid KBSH-44 by amplifying allele of size 230 bp a female specific (CMS-17A) allele and 200 bp amplicon a male specific allele (RHA 95-C-1). SSR primer ORS 811 found specific to identify KBSH-53 and it amplified allele of size 270 bp in its female parent (CMS-53A) and allele size of 230 bp in its pollen parent (RHA 95-C-1). The hybrid has both the alleles from its parents at 270 and 230 bp.

IDENTIFICATION OF SSR MARKERS FOR HYBRIDITY AND SEED GENETIC PURITY TESTING IN SUNFLOWER (Helianthus annuus L.)