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In sunflower, commercial hybrid breeding is based on a single CMSinducing cytoplasm, the so-called PET1 cytoplasm. The introduction of one dominant, nuclear-encoded restorer gene ( Rf1 ) is in most cases sufficient for fertility restoration. Little has been learned so far about the mode of action of the restorer gene  Rf1 . For map-based cloning of the restorer gene  Rf1 , an F 2  population of the cross RHA325 (cms) x HA342 has been used. The χ²-test confirmed segregation for one dominant gene which corresponds to  Rf1 . For the AFLP analyses 256  Eco RI/ Mse I primer combinations have been used so far. In addition, RAPD analyses were performed using 1,200 decamer primers. Twenty-three primers had polymorphic amplification products, differentiating the bulks, and could therefore be mapped. The hybridization of the marker HP4 against a BAC library resulted in three positive clones. The overlapping end of the smallest clone was used to get a new hybridization against the BAC library.

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MAPPING OF THE RESTORER GENE Rf1 IN SUNFLOWER (Helianthus annuus L.) / CREACION DE MAPA DEL GEN RESTAURANTE Rf1 EN EL GIRASOL (Helianthus annuus L.) / CARTOGRAPHIE DU GÈNE Rf1 RESTAURATEUR DE FERTILITÉ CHEZ LE TOURNESOL (Helianthus annuus L.)