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The process of plant breeding and conservation of gene pool among other   things depends on the knowledge of the level of genetic differentiation. The   aim of research in this paper was to determine the genetic differentiation   of silver fir (Abies alba Mill.) populations of regular type and atypical   genotypes with pyramidal crown that can be found on Javor mountain, at the   site Ogorijevac. The genetic differentiation of silver fir was determined   using SSR (Simple Sequence Repeat) markers. Nine SSR pairs of primers gave   29 alleles, while the average number of alleles was 3.2. The primer NFH15   gave the smallest number of alleles (two), while the primer SF78 gave the   greatest number of alleles (five). Dice coefficient of the genetic   similarity was used to obtain a dendrogram by UPMGA analysis using NTSYSpc   statistical program. The genetic similarity recorded among the individuals   P1 and P2 was the largest (0.89), while the populations VI and individual P2   showed the lowest similarity (0.61).  Based on the cluster analysis it can   be concluded that the studied populations and genotypes of silver fir with   different types of crown are clearly differentiated. The basic insight into   the level of the genetic diversity of the natural populations of silver fir   with the various types of crown has been provided using selected SSR   markers. The obtained results can be used for creating further strategy for   the conservation of the available gene pool and the regeneration of silver   fir forests in Serbia.

Application of SSR markers for assessment of genetic differentiation of silver fir (Abies alba Mill.) originating from Javor mountain