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The objectives of this investigation was to demonstrate that genotypes of   calpastatin (CAST) Ser368Arg and ryadonide receptor 1 (RYR1) g.1843C&gt;T may   affect the carcass traits of pigs. Association analysis of the   aforementioned SNPs was performed on 518 pigs included eight commercial   crossbreeds and one pure pig breed. All pigs were slaughtered at average   body weight of 113kg. Genotypes at the SNPs were determined by PCR-RFLP.   There were found only two genotypes of RYR1 gene: CC (428 pigs) and CT (90   pigs) in this study. The effect of allele C compared to allele T on the   higher fat content of pig carcass was confirmed. The allelic frequencies of   allele 638Ser (C) and allele 638Arg (A) were 0.26 and 0.74, respectively.   The significant association (P&lt;0.05) was occurred between allele A and   higher fat content, and between C allele and higher lean meat content. Our   results showed no significant effect of the observed polymorphismSer638Arg   on the intramuscular fat content or other indicators of carcass value.

Effect of the Ser638Arg variation in the cast gene and causal SNP g.1843C>T in the RYR1 gene on carcass traits in crosbreed pigs