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Certain in vivo studies have shown that the application of adhesives directly   onto the open pulp or on a thin layer of dentin causes inflammation and   pulpal abscesses. This reaction is related to toxic effects of monomers from   adhesives. It has been confirmed that after proper illumination the adhesives   become less toxic. The aim of the study was to examine genotoxicity of   non-polymerised, partly polymerised and polymerised adhesives on isolated   human lymphocytes using the alkaline Comet assay. Adper Single bond2 and   Adper Easy One/3M ESPE adhesive photopolymerisation was performed by Elipar   Highlight 3M ESPE halogen lamp for 0, 10 and 40 sec, at final concentrations   of 100, 200, 500 and 1000 μg/mL. With both adhesives, photopolymerisation at   0 and 10 seconds showed statistically significant increase in DNA damage in   comparision to the negative control (solvent). On the other hand, after 40   seconds of photopolymerisation of both adhesives in all tested   concentrations, the degree of DNA damage in Comet assay had no significant   difference (P>0.05, χ2 test) compared to the negative control. Therefore,   only the 40 seconds of photopolymerisation prevented genotoxic effects of   both adhesives in the Comet assay. [Projekat Ministarstva nauke Republike   Srbije, br. III46002 i br. OI173012

Effects of photopolymerisation on genotoxicity of composite adhesives in the comet assay