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Many of the previous studies indicated that the I/D polymorphism of ACE gene   is associated with diabetes type-2. To validate the association of I/D   polymorphism in ACE gene, a study was designed in non-diabetic (normal) and   diabetic type-2 patients of unrelated Gujarati population. The random blood   samples from 36 normal and 36 diabetic type -2 patients of above 45 years   were collected for the studies. DNA was extracted from blood samples for PCR   by using ACE specific primers. The gene and genotype frequencies were   estimated for different alleles observed in diabetic as well as in normal   healthy persons. In present study, all three genotypes that is, I/I (477bp),   I/D (477/190bp), D/D (190bp) were observed in samples from normal and   diabetic patients. Among all genotypes ID (58.3%) has maximum genotypic   frequency in diabetic than Non diabetic individuals (44.4%), frequency of II   (27.7%) is more in Non diabetic individuals than Diabetic individuals (19.4%)   and genotypic frequency of DD (27.7%) is more in Non diabetic than Diabetic   individuals (22.22%). The results were not in agreement with so many previous   studies. However, recent findings of other studies conducted in different   ethnic groups are similar to our findings which do not support that I/D   polymorphism are associated with type-2 diabetes

Comparative study of I/D polymorphism of ace gene in diabetes type-2 patients and control group in unrelated Gujarati population