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Red clover (Trifolium pratense L.) is one of the main forage species from   temperate regions and it's originated in Eurasia where wild-type populations   are found in the Caucasus Mountains. Considerable variation for this crop has   been recorded for few molecular variations. The identification and   understanding of molecular genetic diversity in red clover accessions will   help in effective genetic conservation along with efficient breeding programs   in this crop. This study has shown molecular diversity using microsatellite   markers in red clover accessions from around of the world. In this   investigation, 40 genotypes have been selected and analyzed using 15 SSR   primers. These primer pairs amplified 1146 polymorphic loci among the   genotypes screened. The number of fragments amplified by each SSR primer   combination varied from 24 for RCS3681 to 109 for RCS1729 with an average   value of 80.78 per primer combination. The SSR marker data was further   analyzed using cluster algorithms and Principal coordinates analysis (PCoA).   The results indicated that the considerable genetic variations were   discovered among the analyzed genotypes. The SSR based clustering could   identify the putative pedigree types of the present red clover types of   diverse origins

Enetic diversity in red clover (Trifolium pratense L.) using SSR markers