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Maize protein is deficient in two essential amino acids, lysine and   tryptophan. Naturally occurring opaque2 (o2) mutation which increases the   levels of tryptophan and lysine in the grain also confers an undesirable   phenotype leading to low yields and more susceptible, soft and chalky   kernels. Quality Protein Maize (QPM) is agronomically acceptable and   nutritionally improved opaque2 maize. Marker assisted selection increases   reliability and efficiency, reduces time and costs taken to obtain QPM. The   results presented in this paper are a part of the Maize Research Insitute   project on conversion of normal maize lines to QPM lines adapted for growing   in temperate regions trough marker assisted backcross (MAB). Genetic and   biochemical variability was analyzed between normal (ZPL 5) and QPM (CML 144)   parental inbred lines and the efficiency of the three opaque2 specific SSR   markers was examined. Markers phi057 and umc1066 can discriminate homozygous   and heterozygous backcross progeny, thus can be used as foreground selection   markers for the opaque2 gene. The genetic similarity between analyzed lines   was 0.05, which confirmed good selection of parental lines for the creation   of hybrids. Tryptophan content was 0.093 and QI was 0.85 in CML 144,   approximately twice as high compared to 0.054 and 0.48 in ZPL 5. Both   tryptophan and QI values for CML 144 were above the QPM treshold, i.e.   tryptophan for 24% and QI for 0.625%, making it a suitable donor line of o2.   These results represent the framework for marker assisted introgression of   the quality protein trait into local maize genotype. [Projekat Ministarstva   nauke Republike Srbije, br. TR31068: Improvement of maize and soybean   characteristics by molecular and conventional methods

Genetic and biochemical characterization of parental inbred lines in marker assisted selection for quality protein maize