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In the present study polymorphism of the exon 2 of MHC (Major   Histocompatibility Complex) gene in Makuie sheep breed was studied. Genomic   DNA from blood samples of 90 sheep was extracted and a 279 bp MHC exon 2   fragment was amplified using polymerase chain reaction (PCR). PCR products   were subjected to enzymatic digestion using RsaI endonuclease. Digested PCR   products were electrophoresed on 2% agarose gel. The results showed the   existence of 10 alleles: A, B, E, F, I, M, O, P, Q and V for the exon 2 of   the MHC gene, with the frequencies of 0.4756, 0.0976, 0.0183, 0.0366,   0.0549, 0.0122, 0.1098, 0.0915, 0.0854 and 0.0183, respectively. Eighteen   genotypes: AA, AB, AE, FF, AM, BO, EO, IO, OM, AP, BP, OP, PP, AQ, OQ, PQ,   QQ and AV with the frequencies of 0.317, 0.1585, 0.0121, 0.0365, 0.0121,   0.0243, 0.0243, 0.1097, 0.0121, 0.0487, 0.0121, 0.0365, 0.0365, 0.0487,   0.0121, 0.0121, 0.0487 and 0.0365, respectively were identified in the   population under study. Effective number of alleles and heterozygosity for   the examined region were 3.7231 and 0.7314, respectively. Chi-square test   showed that the examined sheep population was not in Hardy-Weinberg   equilibrium in the examined region

Study on genetic variability in MHC-DRB1 second exon in Makuie sheep breed population