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Four inbred lines of maize (Os 438-95 = C1, Os 30-8 = C2, Os 6 = C3 and Os   1-44 =C4) were grown for 4-year period (2006-2009) in the stationary field   experiment on Osijek eutric cambisol. Impact of irrigation, nitrogen   fertilization and genotype were tested. Soil moisture was maintained by two   irrigation rates from 60-100% and 80-100% of the field water capacity). Two   steps of N (0, 100 and 200 kg N ha-1) were applied, while P and K   fertilization was equal (500 kg/ha NPK 0:30:20). Eight maize genotypes (four   inbred lines and four hybrids) were grown on each basic plot of   fertilization. The experiment was duplicated for maize - soybean rotation.   The experiment was set by split-split plot method according to randomized   block design in three replicates. The basic plot areas were 617.2 m2   (irrigation), 313.6 m2 (fertilization) and 39.2 m2 (genotype). Selection of N   non-fertilized treatment and four inbred lines were made for this study with   aim of testing year (A) irrigation (B) and genotype (C) effects under natural   N-soil conditions. Average grain yield in level 1809 kg ha-1without N   fertilization is indication of very high fertility of the soil. Differences   of yield among the years were from 823 (2007) to 2450 (2006) kg ha-1.   Excessive drought and high air-temperature stress is responsible for the low   maize yield in 2007. Irrigation considerable affected on maize yields (4-year   averages: 1500, 1809 and 2118 kg ha-1, for B1, B2 and B3, respectively).   Differences of the 4-year average yields among the genotypes were from 1259   (C3) to 2765 (C1) kg ha-1. Differences of yield among the genotypes in the   different years were also considerable because the lowest yield was for 71%   (A1), 23% (A2), 63% (A3) and 40% (A4) lower in comparison to the highest   yield. The genotype effects under different water supplies were less   influencing factor because the high-yielding C1 had for 128%, 129% and 106%   the higher yield compared to the low-yielding C3, for B1, B2 and B3,   respectively. Differences of grain -protein, -starch and -oil among the years   was from 9.61 to 11.84%, from 68.51% to 70.93% and from 3.50% to 4.17%,   respectively. The C2 separated by the higher grain protein contents (10.93%)   from the remaining three genotypes (average 9.96%). The genotype effects on   starch and oil contents were from 69.83% (C4) to 70.58% (C1) and from 3.56%   (C3) to 4.09% (C1), respectively

Impacts of irrigation and genotype on yield, protein, starch and oil contents in grain of maize inbred lines