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Optimization of the growth condition for maximum growth rate and protease  production was carried out using Bacillus subtilis. The optimization of  protease production using agro industrial waste product such as cassava waste  as substrate was performed with statistical methodology based on experimental  designs. The screening of twelve nutrients for their influence on protease  production was achieved using a Plackett-Burman design. MgSO4.7H2O, casein  and glucose were selected based on their positive influence on protease  production. The selected components were optimized using Response Surface  Methodology (RSM). The optimum conditions are (% w/w): MgsO4.7H2O- 0.14,  casein- 1.4 and glucose- 2.64. These conditions were validated experimentally  which revealed an enhanced protease yield of 202.048 U/gds.

Response surface optimization of medium composition for protease production by Bacillus subtilis using cassava waste