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For the purpose of detecting methicillin (oxacillin) resistance in  staphylococcal strains, in a number of microbiological laboratories only disc  diffusion method with cefoxitin and/or oxacillin discs is used. Besides this  method, it is desirable to determine MIC values for cefoxitin and/or  oxacillin. After examination by disc diffusion and dilution methods, latex  agglutination is used for the detection of PBP2a and PCR is used for the  detection of mecA gene. Use of PCR is not possible in a large number of  diagnostic laboratories and as method of choice, latex agglutination test for  rapid detection of PBP2a is recommended. In this investigation, as  confirmatory methods, latex agglutination and PCR were used for strains that  were resistant to oxacillin and/or cefoxitin by disc diffusion and broth  microdilution methods. In total, 14 strains of coagulase-negative  staphylococci originating from clinical specimens of cats, dogs and chicken  were examined. Among isolated strains, it was established that the dominating  species was Staphylococcus haemolyticus with 11 isolated strains. Other  isolated species were Staphylococcus epidermidis, Staphylococcus capitis and  Staphylococcus vitulinus, each with one isolated strain. For all 14 strains,  oxacillin MIC values ranged from 0.5 μg/mL to &gt;64 μg/mL and cefoxitin MIC  values ranged from 1 μg/mL to &gt;256 μg/mL. Positive agglutination reaction by  latex agglutination test was recorded in 13 out of 14 strains. The PCR assay  for mecA gene was positive in 12 investigated strains. [Projekat Ministarstva  nauke Republike Srbije, br. 31079

Detection of PBP2a (penicillin-binding protein 2a) and mecA gene in methicillin resistant staphylococci originated from animals