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Limbaugh?s damselfish, Chromis limbaughi Greenfield &amp; Woods, 1980, is endemic   to the Gulf of California, and one of the five most exploited species for   the aquarium trade in this region. C. limbaughi is a gonochoristic,   gregarious and territorial species without sexual dimorphism that inhabits   rocky, sheltered areas. Development of captive breeding techniques for this   species would not only ensure a continued supply of fish for the commercial   trade, but perhaps more importantly, it would also alleviate fishing   pressure and support stock enhancement. Thus, as a first step towards   achieving these goals, in this work, we investigated some aspects of the   reproductive biology of C. limbaughi. Seasonal fish samplings, with a total   of eighty-nine fish caught in one year, were carried out at San Esteban   Island, Gulf of California, Mexico. The reproductive season of C. limbaughi   extends, at least, from May to September. A new maximum standard length of   10.5 cm is reported for this species. The estimated size at first sexual   maturity was 7.90 cm for males and 7.59 cm for females. For both male and   female gonads, the major constituent fatty acids were palmitic acid, stearic   acid, oleic acid, docosahexaenoic acid, eicosapentaenoic acid and   arachidonic acid. The water-quality conditions under which maturation of C.   limbaughi took place were measured, and should prove useful for the   management of broodstock in captivity.

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Seasonal changes in gonad maturity, proximate and fatty acid composition of Limbaugh’s damselfish, Chromis limbaughi Greenfield &amp; Woods, 1980 (Pisces: Pomacentridae)