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Titulo en ingles : Identification of Leishmania ( Viannia ) guyanensis in dogs in rural area municipality of Villavicencio, Meta, Colombia. RESUMEN:  La leishmaniasis cutanea canina es una enfermedad emergente en Colombia. Aunque ha sido posible establecer que el canino pueden ser parasitado por especies de Leishmania ( Viannia ) de alta importancia epidemiologica como L. panamensis y L. braziliensis. , aun no se ha determinado su papel en la transmision de la Leishma- niasis cutanea americana (LCA). Se tomaron muestras para estudios moleculares en la vereda La Reforma del Municipio de Villavicencio, en donde se habian encontrado 6 caninos y un humano con lesiones compatibles con Leishmaniasis cutanea. Las muestras de tejido fueron recolectadas y procesadas para la identificacion del parasito a traves de la tecnica de Reaccion en Cadena de la Polimerasa (PCR). Se utilizaron los iniciadores OL1 y OL2 para la identificacion del parasito, los iniciadores B1 y B2 para determinar el Sub-genero y los iniciadores especie-especificos b1-b2, g1-g2, y p1-p2 para la especie. Se encontro en el humano y un canino un patron de amplificacion de ADN compatible con el reportado para Leishmania (Viannia) guyanensis . En conclusion, la identificacion de esta especie de Leishmania fue posible mediante PCR, lo cual valida su uso en estudios futuros y ademas sugiere la existencia de un nuevo nicho ecologico para el parasito lo que representa un importante factor de riesgo para la presentacion de un brote epidemico en la poblacion. Palabra s clave: Leishmaniasis cutanea, caninos, Villavicencio SUMMARY:  The canine cutaneous leishmaniasis is an emerging disease in our country.Although it has been possible to establish that the canines can be parasitized by species of Leishmania ( Viannia ) high epidemiological importance such as the L. panamensis and L. braziliensis . not yet determined its role in the transmission of American cutaneous leishmaniasis (ACL). The taking of samples for molecular study was conducted at the village La Reforma of municipal of Villavicencio, where identified a 6 canines and one humans with lesions consistent with cutaneous leishmaniasis. The tissue samples were collected and processed for identification of the parasite through the PCR technique. We used the primers OL1 and OL2 for identification of parasites of the genus Leishmania, the B1 and B2 primers to determine the sub-genus and species-specific primers b1-b2, g1-g2, and p1-p2 to determine the species.Achieved the identification of a canine and a human whose samples showed a pattern of amplification of DNA compatible with the reported for Leishmani a (Viannia) guyanensis . In conclusion, the technique of PCR is a feasible diagnostic technique for the diagnosis of leishmaniasis and the identification of species parasitante in each case. The identification of a Leishmania species from sylvan distribution in rural area of Villavicencio implies the existence of a new ecological niche for the parasite that represents an important risk factor for the generation of an epidemical outbreak in the population. Key words: cutaneous leishmaniasis, canine, Villavicencio

Identificación de Leishmania (Viannia) guyanensis en caninos, en zona rural del municipio de Villavicencio, Meta, Colombia