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Resumen Solanum goniocalyx Juz. & Bukasov (Solanacea), comunmente llamada “papa amarilla”, presenta muchas variedades comerciales como amarilla, Tumbay y peruanita, y tiene una distribucion geografica amplia tan vasta como la papa comun. Se propaga vegetativamente a traves de tuberculos como semilla, lo cual proporciona ventajas manteniendo caracteristicas de una determinada variedad, pero tambien existen desventajas como la transferencia de enfermedades y no poseer, en el mayor de los casos, buenas condiciones geneticas. Estos problemas han hecho necesaria la busqueda de alternativas de propagacion y han permitido la aplicacion de nuevos metodos como el cultivo in vitro de tejidos vegetales. Dentro de las tecnicas de cultivo in vitro de tejidos vegetales que se han desarrollado en papa, se tiene a los cultivos a partir de meristemos, brotes axilares, embriogenesis somatica, etc. Por lo tanto, el objetivo de la presente investigacion fue inducir a la formacion de callo embriogenico in vitro a partir del cultivo de anteras en “papa amarilla”, en el cual se utilizaron cinco tratamientos de medio de cultivo MS, a los cuales se les adiciono citoquininas (zeatina) y auxinas (2,4-D y AIA) en diferentes concentraciones. Se analizo el porcentaje de contaminacion, oxidacion y de formacion de callos embriogenicos. Se determino que el metodo de desinfeccion permite tener 100 % libre de contaminacion, ademas de que las anteras son muy propensas a la oxidacion y que el tratamiento D permitio obtener mas formacion de callos embriogenicos, ademas de que estos presentaron un mayor tamano en comparacion a los demas tratamientos. Palabras claves : Solanum goniocalix Juz. & Bukasov, callo embriogenico , in vitro , anteras, Solanaceae. Abstract Solanum goniocalyx Juz. & Bukasov (Solanaceae), called “yellow potato”, presents many commercial varieties such as amarilla, Tumbay and peruanita; and it has a wide geographic distribution like the common potato. It propagates vegetatively through tubers as seed, which provides advantages maintaining characteristics of a certain variety, but there are also disadvantages like the transfer of diseases and not to have, in most cases, good genetic conditions. These problems have made it necessary to search for propagation alternatives and and have allowed the application of new methods such as in vitro culture of plant tissues. Among the techniques of in vitro culture of tissues that have been developed in potato, we have cultures from meristems, axillary shoots, somatic embryogenesis, etc. Therefore, the objective of this investigation was to induce the formation of embryogenic callus in vitro from anther culture in “yellow potato”, in which five treatments of MS culture medium were used, to which were added cytokinins (zeatin) and auxins (2.4-D and AIA) in different concentrations. The percentage of contamination, oxidation and formation of embryogenic calluses was analyzed. It was determined that the disinfection method allows to have 100 % free of contamination, besides that the anthers are very prone to oxidation and that treatment D allowed to obtain more formation of embryogenic calluses, besides that they presented a larger size compared to the other treatments. Keywords : Solanum goniocalix Juz. & Bukasov, embryogenic callus, in vitro, anthers, Solanaceae.

Inducción in vitro de callo embriogénico a partir del cultivo de anteras en “papa amarilla” Solanum goniocalyx Juz. & Bukasov (Solanaceae)