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The results showed that the carbon concentration decreased with soil depth in the control and treatment samples. The nitrogen concentration had a tendency to increase in the decomposed litter layer. Nitrogen addition did not show significant effect on carbon and nitrogen concentration. The carbon stable 13 isotope ( C) was about -27‰ in forest cover layer and organic matter layer, while it was as high as -25.3‰ 13 in mineral soil layer. Nitrogen addition had no significant effect on the value of C. The range of nitrogen 15 15 stable isotope ( N) was -1.6 ~ 5.5‰, which increased with the depth of soil. N value increased significantly (P = 0.039) in the decomposed litter layer under nitrogen addition.

Effect of nitrogen addition on carbon and nitrogen stable isotopes in temperate forest litter and soil