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Background: Betel leaf essential oil lotion has been known to have insect repellent properties. A lotion was made based on a pharmaceutical formula from a monograph where patchouli leaf essential oil was added. A lotion preparation was intended to enhance adherence of the formula on the surface of the skin. The purpose of this study was to compare protection percentage of lotion with patchouli oil and without patchouli oil lotion compared to DEET. Methods: This study is an experimental laboratory-based research. All mosquitoes were acquired from Loka Litbang P2B2 Ciamis. The lotion was made up of 4% betel leaf oil with 0.4% Patchouli leaf oil which acted as fi xative. Repellency test was performed using methods recommended by the Commission on Pesticides. Results: The lotion made was stable and met the standard of formulations. The results showed that DEET and the lotion had an average protection of above 90% for 6 hours against Ae.aegypti and Culex sp. mosquitoes. Conclusion: The addition of patchouli oil on betel lotions can increase protection against Ae.aegypti and Culex sp. bites. (Health Science Indones 2014;1:44-8)

Potential topical natural repellent against Ae. aegypti, Culex sp. and Anopheles sp. mosquitoes