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Two experiments were conduoted to study the effect of the consumption of different amounts of immature $renean oak leaves (OL) by beef cattle oi in vitro ruminal fermentation and potential adaptauon ofrumen microorganisms. Atotal oftwelve ruminally cannulated young Brown Swiss bulls were divided in experimental groups that roceived differsnt amounts of OL v?a the ruminal cannula (on average 0,2.5,5.2 and almost 10 kg fresh matter per animal and day). The gas production technique was used to study in vitro fermentation of two substrates (grass hay and O[,) incubated with rumen inocrila derived from each bull. Results suggest not only a dose-dependent negative effect oftannins consumption on ruminal fermentation of common feeds (e.9., grass hay), but also an adaptation of rumen microbial populations from animals receiving moderate amounts of OL. The high level of tannins in the rumen of bulls that received tho highest amount of OL would have exceeded the ability of microorganisms to tolerate or detoxify them.

Effect of immature oak (<i>Quercus pyrenaica</i>) leaves intake on ruminal fermentation and adaptation of rumen microorganisms in cattle