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The effect of vitamin E in feed enriched in rape seed oil or sunflower oil on the concentration of vitamin E in the cow milk was studied by chromatographic assay. The experiment was conducted on forty Holstein-Friesian cows. Addition of sunflower oil to the feed resulted in lowering the concentration in the milk compared with the concentration of vitamin E in the milk of cows fed the ration with rape seed oil. Described GLC procedure provided the new accurate analytical tool for quantification of CLA isomers and other fatty acids in milk and blood plasma of cows.

The use of developed chromatographic techniques to determine vitamin E and conjugated linoleic acid isomers in milk of cows