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The impact of feeding myristic acid (C14:0; 50 g/kg DM) to sheep was investigated in a 6 × 6 Latin square arrangement. C14:0 was supplemented to two control diets (forage:concentrate ratios of 1:1.5 and 1:0.5) which were adjusted to different dietary Ca contents (4.2 or 9.0 g/kg DM). In combination with the concentrate-based diet, C14:0 reduced methane energy loss by more than 50% but reduced Ca availability of the animals while the additional Ca supply improved Ca and energy retention. Supplementing C14:0 to the forage-based diet did not affect Ca availability and resulted in highest feed energy utilization.

Impact of the methane-suppressing feed additive myristic acid on energy and calcium balance of sheep