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The objective of this study was to investigate the effects of disodium malate (6.65 mM) on the in vitro fermentation of two diets (F (40% concentrate) and C (90% concentrate)). Malate treatment increased (P<0.001) dry matter and neutral-detergent fi bre disappearance with both diets. Daily production of propionate and butyrate increased (P<0.001) and the ratio CH4:VFA decreased (P<0.001) by adding malate for both diets. Whereas adding malate to F diet produced an increase (P<0.05) in acetate production, this effect was not observed with C diet. In addition, malate treatment increased (P<0.05) the daily growth of solid-associated bacteria with F diet, but no effects (P>0.05) were detected with C diet. The results seem to indicate that disodium malate stimulated the in vitro fermentation of both diets, but a greater response was observed with diet F.

Effects of disodium malate on microbial growth and rumen fermentation of two diets in Rusitec fermenters