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Grass-clover silage was treated with biological (VTT) and chemical (KemiSile 2000) additives to asses their effects on silage fermentation, aerobic stability and rumen fermentation pattern in a Rusitec system. Sixty days after ensiling, basic parameters were determined in silages. The experimental material was then investigated using a Rusitec system. Each of the additives decreased (P<0.05) the ammonia level in rumen fluid after incubation. Both additives increased (P<0.01) the level of protozoa, which can be explained by a higher level of available nutrients. A higher isovalerate content was found in rumen fluid after fermentation, whereas the valerate content was lower when VTT was added to the ensiling material. The additives improved silage quality, but the biological preservative decreased its aerobic stability.

The stability of silage containing biological and chemical additives assessed using a <i>Rusitec</i> system