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Saturated fatty acids (FAs), positional and geometric isomers of mono-, diand tri-unsaturated FAs containing eighteen carbon atoms were separated on two long HPLC RP-C,g columns. Al l FAs in ovine milk, meat, fat, and intestinal digesta were assayed after pre-column derivatization using UV detection at 256 nm. The content of conjugated linoleic acid (CLA) in processed samples can be determined by using simultaneous UV monitoring at 256 and 235 nm. The presented method is a simple, sensitive and selective analytical tool for routine quantification of FAs and CLA.

Quantification of conjugated linoleic acid and other essential fatty acids in ovine meat, milk, fat, and intestinal digesta