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Two experiments with 96 crossbred pigs each were conducted to study the effectiveness of C o to reduce tissue C u accumulation in pigs. A 2 x 3 factorial arrangement of treatments, two levels of C u (15 and 280 mg/kg) and three levels of added Co (0, 150 and 300 mg/kg), was used in both experiments. The initial body weight was 8.2 kg in experiment 1 and 7.2 kg in experiment 2. Tissue samples were taken at d 35 of experiment 1 and d 14 and d 28 of experiment 2 for mineral analysis. Cobalt failed to alleviate C u deposition in the liver and brain at both dietary C u levels and increased (P^0.05) C u deposition in the liver of the 150 mg/kg Co group. In contrast, increasing dietary Co linearly decreased (P^0.05) C u deposition in the kidney and Zn deposition in the liver. High dietary C u increased (P<0.05) serum C u concentration and C u deposition in the liver, brain and kidney. Copper feeding stimulated (P^0.05) growth only during the first week in experiment 1. Dietary supplementation of 150 and 300 mg/kg of Co greatly depressed (P^0.05) feed consumption and reduced growth rate (P ^ 0.05). In summary, the Cu-Co antagonism is tissue specific and could not be used to prevent liver or brain C u accumulation. In addition, the Co tolerance level for weanling pigs is lower than 150 mg/kg. K E Y W O R D S : copper, cobalt, pigs

Characterization of cobalt-copper antagonism in the study of copper-stimulated growth in weanling pigs