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Drooping wild ryegrass (Elymus nutans Griseb.), an important alpine forage grass, is a perennial, caespitose and self-pollinating species in the genus Elymus L. (Poaceae: Triticeae). It is native to temperate and tropical Asia – from western and central Asia in the west to China and Mongolia in the east, from Russia in the north to India and the Himalayas area in the south (Chen et al., 2009). It grows in grassland, along river banks, among bushes, on mountain slopes and in swales, at altitudes from 1000 m up to 5000 m (Lu, 1993). In China, it is distributed in north, northwest and southwest part of the country, particularly on Qinghai Tibet Plateau (Miao et al., 2011). Drooping wild ryegrass is the valuable forage grass in the alpine regions due to its high adaptability, good tolerance to cold, drought ABSTRACT. The aim of the study was to evaluate the effect of sucrose, formic acid and lactic acid bacteria (LAB) inoculant on chemical composition and in vitro rumen digestibility of drooping wild ryegrass (Elymus nutans Griseb.) silage. Fresh drooping wild ryegrass was harvested at boot stage and ensiled with: 1. control (untreated), 2. 20 g · kg−1 sucrose (S), 3. 3 g · kg−1 formic acid (FA) and 4. 0.005 g · kg−1 commercial LAB inoculant (LP). The addition of all three additives increased the Flieg point, and decreased pH and butyric acid content (P < 0.05). There was lower ammonia N content in silages treated with S and FA. On the other hand, LP addition elevated lactic acid content with simultaneous increase of the lactic to acetic acid ratio (P < 0.001). The silages were further anaerobically incubated at 39 °C for 48 h with buffered rumen fluid of lactating cows. All three additives increased volatile fatty acid production (P = 0.008). Addition of LP increased average gas production rate and ammonia N content (P < 0.001). In brief, well fermented drooping wild ryegrass silages were obtained by adding LP or S in comparison to FA. LP can be recommended as a silage additive for preparing well fermented silage, and FA could be applied as a regulator additive to control the level of lactic acid produced during drooping wild ryegrass ensiling. Received: 15 May 2015 Revised: 7 November 2016 Accepted: 22 February 2017

journal of animal and feed sciences

Effects of sucrose, formic acid and lactic acid bacteria inoculant on quality, &lt;i&gt;in vitro&lt;/i&gt; rumen digestibility and fermentability of drooping wild ryegrass (&lt;i&gt;Elymus nutans&lt;/i&gt; Griseb.) silage