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Present method allows a simple and rapid identifi cation of maize and specifi c transgenic soyabean event in raw materials and processed feed samples. The method combines two aspects: suitable DNA purifi cation and amplifi cation by means of multiplex RT-PCR. Effi ciency and accuracy of this method have been tested and the limit of detection (LOD) reached was of 0.1 ng/μl for one of the species and of 1 ng/μl for the other one. The checking of the method has been realized by means of RT-PCR using the specifi c probes for each one of the systems by means of comparison with certifi ed reference materials. The obtained results showed that with this method it is possible to obtain, in a shorter time, quality values for both: identifi cation and quantifi cation of GMOs, in raw and processed samples.

journal of animal and feed sciences

Suitable method for simultaneous and specific detection of maize (&lt;i&gt;Zea mays&lt;/i&gt; l.) and genetically modified soyabean (&lt;i&gt;Glycine max&lt;/i&gt; l.) in animal feeds