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Steroid hormones, mainly progesterone (P4) and oestradiol-17β (E2), affect oviduct functioning in pigs and other species (Hunter, 1991, 2012; Hunter et al., 1999; Chen et al., 2013). These steroids affect gene expression, cells ultrastructure and differentiation, motility of oviduct, as well as the composition and volume of oviductal fluid during the oestrous cycle (Rodriguez-Martinez et al., 1982; Buhi, 2002; Yaniz et al., 2006; Carrasco et al., 2008; Skowronski et al., 2011; Hunter, 2012). Thus, steroids regulate oviductal microenvironment and support maturation of gametes, fertilization and early embryonic development (Hunter et al., 1999; Wollenhaupt et al., 2001; Buhi, 2002; Georgiou et al., 2005; Brüssow et al., 2008; Chen et al., 2013). The embryos stay in oviduct only for 2 3 days after fertilization (Hunter, 1974), so most of the oviduct physiological adaptations should occur before and around ovulation in pig. Therefore, steroid hormones can be secreted by the oviduct during the oestrous cycle and, as a local regulators, prepare the organ for its physiological functions. ABSTRACT. Steroid hormones regulate the oviductal functions in cyclic pigs. The mRNA and protein steroidogenic enzymes expression – 3β-hydroxysteroid dehydrogenase (HSD3B), encoded by HSD3B and P450 aromatase (P450arom), encoded by CYP19, were studied in ampulla and isthmus of pig oviducts during luteal (days 2 3, 10 11 and 15 16) and follicular (days 18 20) phase. Expression of HSD3B in ampulla was greater on days 2 3, 12 13 and 18 20 vs 10 11 and 15 16. In isthmus it was greater on days 2 3 vs 10 11 and on days 18 20 vs 10 11 to 15 16. Proteins were immunolocalized in epithelial cells of ampulla and isthmus. On days 15 16, HSD3B concentration in ampulla was the greatest, in isthmus it was greater on days 15 16 vs 12 13. The P450arom protein expression was the greatest on days 2 3 in ampulla and isthmus and thereafter it gradually declined toward the end of the oestrous cycle. Progesterone concentration (pg · ml–1) in oviductal flushings did not change from days 2 3 (960.2 ± 367.2) to 15 16 (543.4 ± 235.3), but decreased on days 18 20 (154.5 ± 34.8). Androstenedione concentration (pg · ml-1) on days 2 3 (726.4 ± 108.4) and 18 20 (701.0 ± 59.0), and oestradiol-17β (pg · ml–1) levels on days 12 13 (104.1 ± 9.6) and 18 20 (110.4 ± 14.4) were greater than on other days. This study showed, that expression of mRNA and proteins of steroidogenic enzymes in oviduct of cyclic pigs depends on the stage of the oestrous cycle. This observation suggests ability of porcine oviduct to de novo synthesize steroid hormones. Received: 2 December 2015 Revised: 12 April 2016 Accepted: 26 August 2016

Expression of 3β-hydroxysteroid dehydrogenase and P450 aromatase in porcine oviduct during the oestrous cycle