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“Greater  wax moth” Galleria  mellonella  L.  (Lepidoptera:  Pyralidae)  is  occasionally  found  in  beehives  and  is  the  mainpest of stored wax. The objective of the present study was to evaluate the pathogenicity of native monosporic strainsof M.  anisopliae  against G.  mellonella larvae. Four  polysporic  indigenous  isolates  were  used  (MaA1,  MaA2,  MaA3,MaA4).  Ten  monosporic  strains  were  obtained  from  each  polysporic  isolate.  Thus,  a  total  of  40  strains  was  obtainedand  used for  the  pathogenicity assay.  Monosporic  strains  were  formed  into  four groups  based  on parental  polysporicisolates.  Statistical  analysis  applied  separately  to  each  of  the  four  groups  of  monosporic  strains  showed  significantdifferences  in G.  mellonella  mortality.  The  more  pathogenic  strains  were  MaA4(5),  MaA1(4)  and  MaA3(7),  withmortality  of  100.0,  88.9  and  83.3  %,  respectively.  The  results  indicate  wide  intra-specific  variability  of  monosporicstrains  of M.  anisopliae in  terms  of  their  pathogenicity  to  larvae  of G.  mellonella.  They  also  demonstrate  the  utilityof  monosporic  strains  and  their  pathogenic  characterization  for  selection  of  isolates  with  outstanding  features;  aswell  as,  the  potential  of  the  studied  fungus  for  development  of  bioinsecticides  against  “greater  wax  moth” G.mellonella.

Cepas monospóricas de Metarhizium anisopliae y su patogenicidad sobre Galleria mellonella en Tabasco, México