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Acetyl-CoA Carboxylase (ACCase) is considered to be one of the key enzymes in palm oil biosynthesis. Availability of genes encoding this enzyme would give some advantages in the molecular breeding of oil palm. Over expression of the genes in the oil palm mesocarp might increase the oil production in this tissue. On the other hand, down regulating of ACCase could divert the central metabolite Acetyl-CoA to other product such as PHB (Polyhydroxy-butyrate), one of the known biodegradable plastic. This paper reported the work of cloning of the full length coding sequence of biotin carboxylase (BC), one subunit of the ACCase. Based on the DNA sequence of the BC conserved region that had been cloned previously, primers pairs were designed to amplify 5’and 3’cDNA ends of BC using RACE-PCR. The RACE products of 5’and 3’cDNA ends of BC were cloned into E.coli, and the DNAs were sequenced and analysed. The full cDNA of BC was obtained by reisolation of the cloned 5’and 3’cDNA ends followed by digestion using KpnI, ligation into pGEM-T vector and cloning into E.coli. Colony PCR was carried out to confirm that the target gene has been cloned. The recombinant plasmid containing full cDNA of BC was then isolated for DNA sequencing. The results showed that the 5’-BC (1367 bp), 3’BC (1032 bp), and the full length cDNA encoding BC (2182 bp) had been successfully cloned, and the DNA sequence had been confirmed as gene encoding ACCase subunit biotin carboxylase. [

Kloning cDNA lengkap penyandi ACCase subunit biotin carboxylase dari mesokarp kelapa sawit (Elaeis guineensis Jacq.) Cloning of full length cDNA encoding ACCase subunit biotin carboxylase from mesocarp of oil palm (Elaeis guineensis Jacq.)