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Spirulina platensis is a blue-green microalga which is frequently used for food and feed supplements and cosmetic active agent. This microalga also produces a strong antioxidant namely superoxide dismutase (SOD) used as cosmetic active agent for anti aging and anti free radicals. SOD was isolated from S. platensis cell biomass from local isolate grown on latex serum on semipilot (3.5 m 3 ) and pilot scale (40 m 3 ) then dried with spray drying or sun drying and characterized. SOD was purified with sequential two-stage sedimentation using ammonium sulphate and fractionated in chromatographic column containing Sephadex G 200. The fractions were analysed to determine the activity, cofactor metal and amino acid composition of the antioxidant. The results showed that sedimentation of SOD extract with 80% ammonium sulphate produced SOD with higher activity compared to that of SOD from commercial S. platensis biomass. This SOD was successfully isolated and purified. Metaloenzyme SOD was composed of subunits with molecular weight of 77.78; 71.74; and 19.2 kDa, which contained nine types of amino acids with tyrosine and lysine as the major amino acid components. Zn was the most predominant metal on SOD, then followed by Fe and Mn. The main subunit cofactors consisted of Zn 72%, Fe 25%, Mn 2%, and Cu 1%, which were different from the small subunit that contained of Zn 55%, Mn 31%, Fe 14%, and Cu 4%. The stability of SOD was achieved on pH 7.5 and temperature below 25 o C. Abstrak Spirulina platensis adalah mikroalga hijau biru yang banyak digunakan sebagai suplemen pangan, pakan, dan bahan aktif kosmetika. Mikroalga ini juga menghasilkan antioksidan kuat yaitu superoksida dismutase (SOD), yang merupakan bahan aktif kosmetika anti penuaan dini dan pencegah efek radikal bebas. SOD diisolasi dari biomassa sel S. platensis isolat lokal yang dibiakkan dalam serum lateks skala semipilot (3,5 m 3 ) dan pilot (40 m 3 ) serta dikeringkan dengan cara pengeringan kabut (spray drying) atau penjemuran untuk kemudian dikarakterisasi. SOD dimurnikan dengan peng- endapan bertingkat menggunakan ammonium sulfat dan dipisahkan dengan kolom kromatografi berisi Sephadex G 200. Hasil pemisahan kemudian dianalisis untuk menentukan aktivitas, logam kofaktor serta komposisi asam amino anti oksidan tersebut. Hasil penelitian menunjukkan bahwa pengendapan ekstrak SOD dengan SOD lebih tinggi dari SOD asal biomassa S. platensis komersial. SOD tersebut telah berhasil diisolasi dan dimurnikan. Metaloenzim SOD tersusun atas subunit dengan BM 77,78; 71,74; dan 19,2 kDa, yang mengandung sembilan jenis asam amino dengan tirosin dan lisin sebagai komponen asam amino utama. Logam yang dominan pada SOD adalah Zn, disusul kemudian Fe dan Mn. Kofaktor sub unit besar terdiri dari Zn 72%, Fe 25%, Mn 2%, dan Cu 1%, berbeda dengan sub unit kecil yang mengandung Zn 55%, Mn 31%, Fe 14%, dan Cu 4%. Stabilitas SOD S. platensis dicapai pada pH 7,5 dan suhu di bawah 25 o C ammonium sulfat 80% menghasilkan aktivitas

Produksi, isolasi dan karakterisasi superoksida dismutase dari Spirulina platensis yang dibiakkan dalam serum lateks Production, isolation, and characterization of superoxyde dismutase from Spirulina platensis cultured on latex serum