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Summary Promoter is a regulator of gene expression for a phenotype or trait carried by the gene. In the structure, a promoter located beyond the 5’ end of the open reading frame of the gene on which its expression is regulated. This research aimed to isolate the DNA fragment flanking TcLFY at the 5’ end and to analyze whether the fragment has charac- teristics of the promoter, primarily the core motifs of promoter. Using Genome Walking technique, DNA fragments flanking the TcLFY gene at its 5’ end was isolated. Analysis of the DNA sequence was done using online computer software accessible through web site www.softberry.com and an entry sequence of the flanking DNA fragment along with the 2.5 kb TcLFY sequence. The result indicated that the flanking fragment has core motifs for a promoter at proper positions, which are TATA box at position –80, CAT boxes (CCAAT) at - 387 and –626, and GC boxes that are known as UAS were found at the -323 and –537 positions. To obtain a conclusive result, this promoter sequence needs to be further examined to confirm its function. Ringkasan Promoter merupakan pengendali ekspresi gen untuk memunculkan fenotipe atau karakter yang dibawa oleh gen tersebut. Di dalam strukturnya, promoter umumnya terletak di daerah ujung 5’ gen yang dikendalikan ekspresinya. Tujuan penelitian ini adalah mendapatkan fragmen DNA yang mengapit gen pengendali pembungaan kakao (TcLFY) dan menganalisisnya apakah memiliki karakteristik promoter, yaitu mengandung motif-motif inti (core motifs) dari promoter. Dengan teknik Genome Walking, fragmen DNA pengapit gen TcLFY di ujung 5’ dapat diisolasi. Analisis sekuen menggunakan perangkat lunak komputer online (www. softberry.com) dengan input data fragmen tersebut ditambah gen TcLFY 2,5 kb di bawahnya, mengindikasikan adanya beberapa motif inti promoter pada posisi yang sesuai, yaitu kotak TATA pada lokasi –80, kotak CAT (CCAAT) di posisi -387 dan –626, dan kotak GC yang merupakan UAS dijumpai pada lokasi -323 dan –537. Untuk memperoleh hasil yang bersifat konklusif, sekuen promoter ini masih perlu diuji fungsinya.

Identifikasi dan isolasi promoter gen pembungaan kakao TcLFY Identification and isolation for promoter of TcLFY cacao flowering gene