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Heteromeric acetyl-CoA carboxylase (htACCase) is one of key enzymes in palm oil biosynthesis. Isolation and characterization of the gene is an important step in metabolic engineering to increase palm oil content and quality. The objective of this research was to isolate DNA fragment of gene encoding biotin carboxyl carrier protein (BCCP) subunit of htACCase from three different oil palm types (Simalungun, Hibrida and Backcross) and investigate the variation of its DNA sequence. Total RNA was isolated from the mesocarp of oil palm. DNA fragment encoding BCCP was amplified by means of Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) using specific primers with total RNA as a template. The products of RT-PCR were then purified from the gel, cloned and sequenced. The DNA sequences were analyzed for their homologies to BCCP gene using BlastN and aligned to detect the sequence variability using ClustalW program from BioEdit. The results show that one of the two RT-PCR products at about 300 bp was highly homologous with the gene encoding BCCP from Glycine max, Brassica napus and Arabidopsis thaliana. Nucleotide sequences of that BCCP fragments from the three types of oil palm displayed some degrees of variability. Further investigation is needed to analyze the variability of the DNA sequences of the full-length gene in relation with oil content or other characters. [

Keragaman sekuen DNA fragmen gen penyandi ACCase subunit BCCP dari tiga tipe kelapa sawit Variability of DNA sequence of gene fragment encoding BCCP subunit of ACCase from three types of oil palm