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Summary Pod rot is one of the most important diseases in cacao. This disease could be incited by Phytophthora palmivora, P. megakarya , P. capsici or P. citrophthora . The causal agent of pod rot disease in cacao in Indonesia is known to be P. palmivora . The success of pod rot disease management is partly depend on the success of efforts in reducing the quantity and quality of the disease inoculum above and below soil surface. Provision of molecularbased detection system would improve the accuracy of determination of these two parameters. The objective of this experiment was to develop a pair of primers that could be used to specifically amplify rDNA fragments of P. palmivora associated with pod rot disease in cacao. Design of these primers was made based on the DNA sequence of rDNA fragment amplified using a pair of universal primers ITS4/ITS5. Regions showing high degree of dissimilarity among species of Phytophthora and high degree of similarity within the same species of P. palmivora were determined through DNA alignment. Specific forward primer (DTF) 5 ′-CTT AGT TGG GGG TCT CTT TC-3′ and reverse primer (Ilyas1R) 5 ′GTT CAC CAA TCA TAC CAC C-3′ were obtained. This pair of primers had been proven to specifically amplify only rDNA fragment, approximately 650 bp, of P. palmivora associated with pod rot disease and stem canker in cacao.

Pengembangan penanda molekuler untuk deteksi Phytophthora palmivora pada tanaman kakao Development of molecular marker for the detection of Phytophthora palmivora in cacao