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Genetic similarity among ten rubber clones originating from the Wickham collection was studied by Amplified Fragment Length Polymorphism (AFLP) markers. These clones have different levels of resistance to Corynespora cassiicola, one of the major pathogens in rubber plantations. The information resulted from this study will be used to determine resistant and susceptible clones which will be used in expression study of the genes encoding plant resistancy to C. cassiicola. Genetic similarity values of clones were calculated from all AFLP markers and used to produce a dendrogram using Unweight Pair-Group Method Arithmetic (UPGMA) based on Numerical Taxonomy and Multivariate System (NTSYS) version 1.8 pc. A total of 481 fragments were detected by using ten pairs of selective AFLP primers, and 233 fragments (48,4 %) of them were polymorphic. The results clearly demonstrated that genetic back-grounds of these ten clones were 85.5% similar. At 88.0% similarity level, the clones could be divided into three clusters. Genetic similarity of IRR 100 (resistant clone) with RRIC 103, PPN 2444 and IAN 873 (susceptible clones) was 90.5, 89.5 and 89.0% respectively. While genetic similarity of other three resistant clones (AVROS 2037, PR 255 and BPM 1) to those susceptible clones was 88.0%. The lowest genetic similarity (85.5%) was found between RRIC 100 (resistant clone) and those three susceptible clones. By considering the distribution and the source of clones, AVROS 2037 (resistant) and PPN 2444 (susceptible) clones which have 88.0% genetic similarity were finally will be selected for the expression study of the genes.

Kemiripan genetik klon karet (Hevea brasiliensis Muell. Arg.) berdasarkan metode Amplified Fragment Length Polymorphisms (AFLP) Genetic similarity of rubber clones (Hevea brasiliensis Muell. Arg) based on Amplified Fragment Length Polymorphisms (AFLP) method