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Coffee skin and pulp compromised almost 50–60% of coffee bean, in which most of them will be turned to waste during coffee processing. Until now, the utilization of coffe skin is still limited to low-value products such as compost, fertilizer and feed. Further utilization of coffee skin has been reported by means of chemical or enzymatic degradation of its structure, whether intended to improve the digestibility of feed, improving the nutritional properties or as potential media of fermentation. This research aimed to study the potential of coffee skin fiber to be used as the source of reducing sugar using acid cellulase enzyme and determine the optimum process of hydrolysis of the coffee skin fiber. Delignification using base was carried out prior to enzymatic hydrolysis. Small scale enzymatic hydrolysis was carried out utilizing Sqzyme CSP, an acid cellulase enzyme in various units (200, 400, and 800 units) for 12 and 24 hours. The evaluation of the hydrolysis performance was carried out by total and reducing sugar analysis followed by the quantification using liquid chromatography-mass specthrometer method. The total sugar content was ranged from 0.149 mg/mL to 0.584 mg/mL whereas the reducing sugar content was ranged from 0.013 mg/mL to 0.114 mg/ mL with the highest production of sugar was obtained using 800 unit of enzyme and incubated for 24 hours. The glucose content was 36,1 ppm or compromised around 31.57% of total reducing sugar produced in the best treatment. The study shows that the enzyme used in this experiment could be used in first stage of coffee skin fiber hydrolysis process to obtain abundant substrat for potential subsequent process.

Utilization of coffee skin fiber as potential source of reducing sugar by means on enzymatic hydrolysis