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An experiment entitled “Response of gibberellic acid, cow urine and bio-fertilizers on seedling growth parameters of papaya (Carica papaya L.)” was conducted at Instructional cum Research Farm, College of Agriculture and Research Station, (IGKV), Bemetara, Chhattisgarh, India during the year 2018 and 2019. The experiment was laid out in Completely Randomized Design (CRD) with Seventeen different treatments replicated three times. The papaya seeds were sown in polybags which was filled with common media and treated with three different concentration of GA3 (viz., 50, 100 and 150) and 10 per cent cow urine with addition of bio-fertilizers in media soil. The results showed that the treatment T11 (seed soaking with 150 ppm GA3 + 12 hours + azotobacter) was found best for length of shoots, stem girth, height of sapling, fresh weight of root, number of roots, length of root and dry weight of sapling. While, T15 (seed soaking with cow urine 10 (%) + 12 hours + azotobacter) gave higher number of leaves, maximum fresh weight of shoot and fresh weight of sapling.