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A study was undertaken to investigate the effect of melatonin supplementation on thyroid hormones (T3, T4) and cortisol in buffalo calves under summer stress. Twelve healthy Murrah buffalo male calves of 6 month to 1 year age group were taken for the study. Buffalo calves were divided into control (CG) and treatment (TG) group. In TG, Melatonin (18mg/50 kg BW) was injected subcutaneously, two times at 1 and 20 day. Hormones like T3, T4 and cortisol were estimated by Gamma coat TM (125I) RIA kits. Within and between the groups serum T3 and T4 level did not differ significantly (P>0.05), but higher level of serum T3 and T4 was observed in CG than TG. The significant (P<0.05) difference observed in serum cortisol level between the groups and within CG. In conclusion, melatonin used to have its effect on thyroid hormone response and level of cortisol secretion during summer stress. It decreases T3, T4 and cortisol levels in the animals and helps them to cope with adverse effect of summer stress.

Effect of melatonin supplementation on thyroid hormone and cortisol in buffalo calves under summer stress