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The aim of this work is to establish rapid, simple, precise and economical methods viz., area under curve were developed and validated for estimation of tramadol hydrochloride in pharmaceutical dosage forms. Further, this study is designed to validate the developed methods as per ICH guidelines. The work was carried out to for estimation of Tramadol in bulk pharmaceutical form by utilizing area under curve (AUC) method using UV-Visible Spectrophotometry. For this purpose the wavelength range 200-400 nm was selected. Distilled water was used as a solvent throughout the work. Linearity was observed in concentration range 5-25 μm/ml (r2 =0.9992) for the method. The developed method was found to be simple, linear, precise, accurate and sensitive which can be used for routine quality control analysis for spectrophotometric estimation of Active Pharmaceutical Ingredient.

Area Under Curve UV Spectrophotometric Method for Determination of an Opioid Analgesic in Bulk