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Fingerprint profile of bark extract of Nyctanthes arbor-tristis using High Performance Thin Layer chromatography (HPTLC) has been established. HPTLC is a valuable tool for the investigation of medicinal plants with reference to the qualitative analysis of the phytoconstituents. Separation of the active constituents from the extracts has been developed using solvent system of Toluene: Ethyl acetate: Formic acid (5:4:1). The HPTLC analysis showed the presence of the flavonoid quercetin in the standard as well as in the sample and the Rf value was 0.73. These images of fingerprinting help in the proper identification and quantification of the marker compounds. On the basis of the marker compounds, new drugs could be formulated to treat various diseases

HPTLC fingerprinting of stem bark extract of Nyctanthes arbor-tristis (L.)