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A simple, precise, accurate, and economical spectrophotometric method has been developed for simultaneous estimation of emtricitabine(EMB) and tenofovir(TEN) by employing first-order derivative spectrophotometric method. The first derivative spectrum is a plot of the rate of change of absorbance with wavelength against wavelength (dA /dλ versus λ). It is characterized by a maximum, minimum and a cross- over point at the λ max of the absorption band. The linearity was established over the concentration range of 6-16 μg/mL and 9-24 μg/mL for EMB and TEN with correlation coefficients 0.9994and 0.9992, respectively. The mean percentage recoveries were found to be 99.99% for EMB and 100.43%for TEN. The proposed method has been validated as per ICH guidelines and successfully applied for the simultaneous estimation of EMB and TEN in combined tablet dosage form.

journal of drug delivery and therapeutics

VALIDATED FIRST-ORDER DERIVATIVE SPECTROPHOTOMETRY FOR SIMULTANEOUS DETERMINATION OF EMTRICITABINE AND TENOFOVIR DISOPROXIL FUMARATE IN PHARMACEUTICAL DOSAGE FORM