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A simple, fast, specific and accurate reverse-phase high performance liquid chromatography (RP-HPLC) method has been developed and subsequently validated for simultaneous estimation of Aliskiren (ALN) and Felodipine (FLD) from their dosage forms. Agilent 1200 infinity LC equipped with UV-Visible with EZChrom Elite compact version 3.3.2 SP2 software was used. Chromatographic separation was achieved isocratically on a Hypersil BDS C8 column (150A—4.6 mm, 5 I¼ particle size) using a mobile phase, Water (adjusted to pH 3.0 with 10%v/v ortho-phosphoric acid) , Acetonitrile and Methanol in the ratio ofÂ  20:30:50 v/v/v and UV detection was carried out at 254 nm for both the drugs ALN and FLD. The retention time for ALS and FLD was found to be 4.26 and 2.96 min respectively, and recoveries were found between 98 and 102%.Â  The method was found linear over the range of 150-450 Âµg mL-1 for ALS andÂ  5-15 Âµg mL-1 for FLD. The method has been successfully applied to assess the assay of solid dosage formulations.

DEVELOPMENT & VALIDATION OF HPLC METHOD FOR ANALYSIS OF SOME ANTIHYPERTENSIVE AGENTS IN THEIR PHARMACEUTICAL DOSAGE FORMS