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Probiotics are microbial food supplements with beneficial effects on human health. Along with the dietary supplement, probiotics also have immunomodulatory properties. Present study was carried out using the in-vivo utilization of albino mice for the study of immunomodulatory effect of Lactobacillus acidophilus NCDC 195. To estimate the increase immune responses Nitroblue Tetrazolium reduction test, (Inducible Nitric Oxide synthase) test and Phagocytosis (% age) have been studied. Results were compared with the control value and. It was observed that feeding the Swiss albino mice for 15 days with Probiotic dosage (Yogurt), causes 1.98 times increase in Nitroblue Tetrazolium reduction test as compared to control, whereas Inducible Nitric Oxide synthase and phagocytosis shows 1.71 and 1.82 times increase in immune responses respectively. Cell mediated immune response was also calculated, where there was increase of 1.04, 1.07 and 1.02 times in Delayed Type Hypersensitivity (DTH) as compared to control was observed. Keywords: Immunomodulation, Nitroblue Tetrazolium (NBT) reduction, Probiotic, Phagocytosis, Cell mediated immune response, Inducible Nitric Oxide synthetase.

ENHANCEMENT OF IMMUNE RESPONSES BY PROBIOTIC PROPERTIES OF LACTOBACILLUS ACIDOPHILUS NCDC 195