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Cyperus rotundus (Linn.) is an important medicinal plant belonging to family Cyperaceae. Four Flavonoids such as quercetin, kaempferol, catechin and myricetin were isolated from in vivo (leaf  and root) and in vitro callus of this species. These compounds are extensively used in Ayurvedic preparation of medicines and are highly in demand in pharmaceutical industries. The present study therefore was undertaken to determine and compare the production of these flavonoids from in vitro callus culture and in vivo plant parts of C. rotundus . Quantification data revealed that the total flavonoid content (free + bound) was higher in tissue culture than in plant parts. The maximum amount of total flavonoid was found in six weeks old callus tissue (1.96mg/g.d.w) and minimum (0.28mg/g.d.w) in two week old callus tissue. In vivo studies showed higher flavonoids content in leaf in free form (0.58mg/g.d.w.) and bound form (0.48mg/g.d.w.) when compared to root in free form (0.19mg/g.d.w) and bound form (0.11mg/g.d.w). Keywords: Cyperus rotundus , Callus culture, Flavonoids

ISOLATION AND IDENTIFICATION OF FLAVONOIDS FROM CYPERUS ROTUNDUS LINN. IN VIVO AND IN VITRO