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Rapid, isocratic and economical RP-HPLC-PDA method was developed for the simultaneous estimation of Phenylephrine HCl (PEH) and Ketorolac Tromethamine (KTR) in bulk and injectable dosage forms. Chromatographic separation was achieved with the Agilent Eclipse C18 column (150×4.6mm;5μ) and the mobile phase composed of methanol and 2mM Ammonium acetate in the ratio 43:57v/v at a flow rate of 1mL/min. The injection volume was 5μL and eluents were monitored at 220nm. Response was a linear function of concentration in the range 30–105 μg/mL for Phenylephrine HCl (PEH) and 10–35 μg/mL Ketorolac Tromethamine (KTR); the correlation coefficients were 0.999 and 0.999, respectively. The method was validated and is suitable for the simultaneous estimation of PEH and KTR in bulk and injectable dosage forms.

Simultaneous Analysis of Phenylephrine HCl and Ketorolac Tromethamine in Bulk and Injectable Formulations by RP-HPLC-PDA Method