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Objectives: The objective of this study was to investigate the activity of cell proliferation of Hydrolyzed Virgin Coconut Oil (HVCO) and chitosan. HVCO and chitosan concentrations with the highest cell viability were called the best concentrations. The best concentrations of Hydrolyzed Virgin Coconut Oil (HVCO) and chitosan were combined to determine NIH 3T3 cell migration, COX-2 and VEGF protein expression. Design In vitro cell proliferation assay was determined by MTT (Microculture Tetrazolium Technique) assay, cell proliferation activity was measured by calculating cell viability incubated 24 hours, wound closure percentage was tested by scratch wound healing method, expression of COX-2 protein and VEGF protein were measured by immunocytochemical method. Interventions: The variable that was intervened in this study was the concentration of HVCO and chitosan. Main Outcome Measures: The main measurements carried out in this study were the absorbance value of HVCO and chitosan which was converted into viability cell, proliferation activity, percentage of wound closure, and percentage of COX-2 and VEGF protein expression. Results: Cell proliferation activity of HVCO and chitosan resulted the best concentration at 31.25 μg/ml, scratch wound healing assay from the combination HVCO and chitosan resulted the best migration of fibroblast cells at ratio of 50:50 with HVCO 62.5 μg/ml and chitosan 62.5μg/ml. The combination of HVCO and chitosan (50:50) increased protein expression of COX-2 and VEGF. Conclusion: Combination of HVCO and chitosan could increase NIH 3T3 cell migration, COX-2 and VEGF protein expression. Combination of HVCO and chitosan had better wound healing activity in vitro than single use.

The Activity of Combination of Hydrolyzed Virgin Coconut Oil and Chitosan Toward Wound Healing Parameters on NIH 3T3 Cells Using in Vitro Methods