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In the study four released varieties and six high yielding turmeric clones were genotyped using fifteen RAPD markers to see band amplification patterns and genetic variations. Jaccard’s coefficient method was used as an estimator of genetic identity and similarity among the genotypes and UPGMA (un-weighted pair group method, arithmetic average) utilized for clustering the genotypes. All the fifteen RAPD primers produced clear polymorphic amplification for the turmeric genotypes used in the study resulting 68 RAPD bands of which 59 bands were polymorphic with average polymorphism of 86.76%. Jaccard’s similarity between genotypes pairs ranged between 0.36 and 0.93 with a mean of 0.62. Prominent difference was observed between the genotypes with BSR-1 and PTS-8. The genotype CL-101 and CL-193 recorded the highest similarity index (0.93) followed by the combination BSR-2 and CL-101 (0.89). The lowest average similarity index was recorded by the variety BSR-1 (0.49) followed by PTS-8 (0.54) and CL-219 (0.58). The RAPD marker result depicted that the use of such molecular marker tool could be a viable option in the presence of a validated marker system developed for identification of the genotypes.

Genotyping of High Yielding Varieties and Genotypes of Turmeric (Curcuma longa L.) using RAPD Markers