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This study shows the use of 4-hydroxy-3(6-methylbenzo [d] oxazol-2-yl) benzenesulfonic acid (HB-9), a fluorescent dye, for the detection and visualization of helminth eggs and protozoan oocysts diagnosed in domestic animals, by epifluorescence microscopy. Eggs of Acuaria spiralis, Strongyloides papillosus, Capillaria spp., Bertiella spp., Strongyloidea and Eimeria spp., were previously identified through the flotation method and used for analysis. The fluorescence of the eggs was visualized in a BEL R Photonics com sistema de Epi-FluorescênciaINV-100 FL Plus..Only eggs of Acuaria spiralis, Capillaria spp., and Bertiella spp. presented fluorescence when visualized by epifluorescence microscopy. The results indicate that the fluorescente dye used in this study permits a easy, clear and selective detection and visualization ofparasitic structures.

Avaliação de corante fluorescente benzazólico para detecção em parasitos diagnosticados por flutuação simples