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The objective of this study was to evaluate the effect of different doses of prostaglandin E2 (PGE2) on the ovulation of prepubertal female mice. Eighteen In BALB/c prepubertal mice were treated with 5 IU of intraperitoneal (i.p.) eCG on day 0. On day 2, the mice were randomly distributed into 6 groups for the i.p. administration of: 1) 0.5 mL of PBS (n = 3), 2) 5 μg of GnRH (n = 3), 3) 25 μg of PGE2 (n = 3), 4) 50 μg of PGE2 (n = 3), 5) 150 μg of PGE2 (n = 3) e 6) 250 μg of PGE2 (n=3). On day 3, the mice were killed and the oviducts were collected for the oocyte count by transillumination technique under stereomicroscopy. There were no difference in the number of ovulated mice and number of ovulated oocyte in the females treated with GnRH and 250 μg de PGE2, and both groups showed differences in comparison with PBS group (P<0.001). The results demonstrated that the PGE2 dose of 250 μg induces the ovulation in prepubertal female mice.

Efeito da dose de prostaglandina E2 na ovulação de camundongos fêmeas pré-púberes: Estudo piloto