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The implementation of reproductive biotechnologies have in orther to improve reproductive rates, favoring the production of genetically superior animals. The oocyte transfer involves aspiration of oocytes from follicles of a mare and transferring for the oviduct of another mare. The in vitro maturation of oocytes is performed when performing aspiration of follicles smaller than the oocytes in not capable of being fertilized. However, there are limitations due to the lack of slaughterhouses to achieve ovaries of mares for studies, also because of the difficulty of performing superovulations in mares. These factors imply difficulties in the advances of studies with equine oocytes. The intracytoplasmic sperm injection in the horse breaks the barrier of the inefficiency of in vitro fertilization resulting in low sperm motility and fertility. Reproductive biotechnology in horses must be studied so that there is an improvement technique. Due to the great importance of the use of reproductive biotechnologies had as purpose of reviewing on follicular aspiration, in vitro maturation and intracytoplasmic injection in mares. Key-word: oocyte, equine, reproduction.

Aspiração folicular, maturação in vitro e injeção intracitoplasmática em éguas